Journal: Current biology : CB

Article Title: HLH-2/E2A Expression Links Stochastic and Deterministic Elements of a Cell Fate Decision during C. elegans Gonadogenesis

doi: 10.1016/j.cub.2019.07.062

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Strains were maintained at 20°C, Experiments were performed at 25°C, unless otherwise indicated, because fluorescent proteins appeared brighter than at 20°C and the lineages progressed faster, making it easier to capture the progression of early gonadogenesis in long-term imaging experiments. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins BD BactoAgar Fisher DF0140-07-4 Calcium chloride, anhydrous VWR 97062-59 Cholesterol MP 101382 Magnesium Sulfate, anhydrous Avantor-Fisher 2506-01 Potassium phosphate, dibasic Avantor-Fisher 4012-05 Tetramisole hydrochloride (levamisole) Sigma-Aldrich L9756-10G Experimental Models: Organisms/Strains GS7434: arIs51 IV This paper N/A GS8513: arTi145 [ckb-3p::mCherry::his-58::unc-54 3 ʹ UTR ] II This paper N/A GS8592: arTi145 II, cross This paper N/A GS8645: hlh-2(ar614) I This paper N/A GS8675: ccIs4251 I; arTi145 II; arTi112 [ckb-3p::mCherry::his-58:: unc-54 3 ʹ UTR ] V This paper N/A GS8686: ccIs4251 I; arTi145 II ; arTi112 V cross This paper N/A GS8812: arTi145 II ; arIs131; arTi112 , cross This paper N/A GS8949: hlh-2(ar623) I This paper N/A GS9013: hlh-2(ar623ar629) I This paper N/A GS9046: hlh-2(ar623) I; pha-1(e2123) III This paper N/A GS9062: hlh-2(ar623) I; arTi145 II; arTi112 V This paper N/A GS9127: hlh-2(ar623) I; arTi145 II This paper N/A GS9128: hlh-2(ar623ar629) I; arTi145 II This paper N/A GS9129: arTi145 II; lin-12(ar624) III This paper N/A GS9130: hlh-2(ar614) I; arTi145 II; lin-12(ar624) III This paper N/A GS9221: arTi145 II; lag-2(ar635) V This paper N/A GS9223: hlh-2(ar614) I; arTi145 II; lag-2(ar635) V This paper N/A GS9232: hlh-2(ar614) I; arIs51 IV This paper N/A Software and Algorithms MATLAB MathWorks R2015a Micromanager [ 31 ] RRID: SCR_000415 ImageJ [ 32 ] https://imagej.nih.gov/ij/ Other Resource website This paper https://github.com/wolfgangkeil/Attner_Keil_et_al_2019_code Open in a separate window KEY RESOURCES TABLE Three hlh-2 alleles were generated using CRISPR/Cas9 as described in Method Details below. hlh-2(ar623) encodes GFP::HLH-2; the tag was added to the N terminus of HLH-2 as addition at the C terminus would potentially compromise its dimerization and function [ 8 , 33 ]. hlh-2(ar614) contains a deletion in a regulatory element, hlh-2prox [ 8 ]. hlh-2(ar623ar629) is a derivative of hlh-2(ar623) in which the same deletion in hlh-2prox as in hlh-2(ar614) was generated using CRISPR/Cas9.

Techniques: Recombinant, Software